TRANSFORMATION BY RAS ONCOGENE INDUCES NUCLEAR SHIFT OF PROTEIN-KINASE-C

被引：22

作者：

CHIARUGI, V

MAGNELLI, L

PASQUALI, F

VANNUCCHI, S

BRUNI, P

QUATTRONE, A

BASI, G

CAPACCIOLI, S

RUGGIERO, M

机构：

[1] Laboratory of Molecular Biology, Institute of General Pathology, University of Firenze, 50134 Firenze

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 173卷 / 02期

关键词：

D O I：

10.1016/S0006-291X(05)80066-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We measured protein kinase C (PKC) activity in normal and ras-transformed Balb/3T3 fibroblasts; cytosolic and nuclear-associated PKC activity was determined either as phorbol ester binding, PKC-dependent phosphorylation of histone III-S, or phosphorylation of endogenous nuclear proteins. Results demonstrate that ras-transformed fibroblasts show down-regulation of cytosolic PKC accompanied by increase of nuclear-associated PKC. These results provide evidence linking transformation to PKC nuclear shift with consequent phosphorylation of nuclear proteins. © 1990 Academic Press, Inc.

引用

收藏

页码：528 / 533

页数：6

相关论文

共 25 条

[1] RAPID ACTIVATION OF PROTEIN KINASE-C IN ISOLATED RAT-LIVER NUCLEI BY PROLACTIN, A KNOWN HEPATIC MITOGEN [J].

BUCKLEY, AR ;

CROWE, PD ;

RUSSELL, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) :8649-8653

[2] IA BINDING LIGANDS AND CAMP STIMULATE NUCLEAR TRANSLOCATION OF PKC IN LYMPHOCYTES-B [J].

CAMBIER, JC ;

NEWELL, MK ;

JUSTEMENT, LB ;

MCGUIRE, JC ;

LEACH, KL ;

CHEN, ZZ .

NATURE, 1987, 327 (6123) :629-632

[3] TRANSFORMATION OF BALB/3T3 CELLS WITH EJ/T24/H-RAS ONCOGENE INHIBITS ADENYLATE-CYCLASE RESPONSE TO BETA-ADRENERGIC AGONIST WHILE INCREASES MUSCARINIC RECEPTOR DEPENDENT HYDROLYSIS OF INOSITOL LIPIDS [J].

CHIARUGI, V ;

PORCIATTI, F ;

PASQUALI, F ;

BRUNI, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 132 (03) :900-907

[4] SYNTHESIS OF DIACYLGLYCEROL DENOVO IS RESPONSIBLE FOR PERMANENT ACTIVATION AND DOWN-REGULATION OF PROTEIN-KINASE C IN TRANSFORMED-CELLS [J].

CHIARUGI, V ;

BRUNI, P ;

PASQUALI, F ;

MAGNELLI, L ;

BASI, G ;

RUGGIERO, M ;

FARNARARO, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (02) :816-823

[5] SIGNAL TRANSDUCTION IN EJ-H-RAS-TRANSFORMED CELLS - DENOVO SYNTHESIS OF DIACYLGLYCEROL AND SUBVERSION OF AGONIST-STIMULATED INOSITOL LIPID-METABOLISM [J].

CHIARUGI, VP ;

MAGNELLI, L ;

PASQUALI, F ;

BASI, G ;

RUGGIERO, M .

FEBS LETTERS, 1989, 252 (1-2) :129-134

[6] POINT-MUTATED P21-RAS COUPLES A MUSCARINIC RECEPTOR TO CALCIUM CHANNELS AND POLYPHOSPHOINOSITIDE HYDROLYSIS [J].

CHIARUGI, VP ;

PASQUALI, F ;

VANNUCCHI, S ;

RUGGIERO, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 141 (02) :591-599

[7] POTENT SELECTIVE INHIBITORS OF PROTEIN KINASE-C [J].

DAVIS, PD ;

HILL, CH ;

KEECH, E ;

LAWTON, G ;

NIXON, JS ;

SEDGWICK, AD ;

WADSWORTH, J ;

WESTMACOTT, D ;

WILKINSON, SE .

FEBS LETTERS, 1989, 259 (01) :61-63

[8] P21RAS-INDUCED RESPONSIVENESS OF PHOSPHATIDYLINOSITOL TURNOVER TO BRADYKININ IS A RECEPTOR NUMBER EFFECT [J].

DOWNWARD, J ;

DEGUNZBURG, J ;

RIEHL, R ;

WEINBERG, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (16) :5774-5778

[9] RAS-TRANSFORMED CELLS - ALTERED LEVELS OF PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE AND CATABOLITES [J].

FLEISCHMAN, LF ;

CHAHWALA, SB ;

CANTLEY, L .

SCIENCE, 1986, 231 (4736) :407-410

[10] RAS-INDUCED C-FOS EXPRESSION AND PROLIFERATION IN LIVING RAT FIBROBLASTS INVOLVES C-KINASE ACTIVATION AND THE SERUM RESPONSE ELEMENT PATHWAY [J].

GAUTHIERROUVIERE, C ;

FERNANDEZ, A ;

LAMB, NJC .

EMBO JOURNAL, 1990, 9 (01) :171-180