QUANTIFICATION OF PITUITARY MEMBRANE-RECEPTOR SITES TO LHRH - USE OF A SUPERACTIVE ANALOG AS TRACER

被引：57

作者：

CONNE, BS

AUBERT, ML

SIZONENKO, PC

机构：

[1] Endocrine Unit, Department of Pediatrics and Genetics, University of Geneva, 1211 Geneva 4, 30, boulevard de la Cluse

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 04期

关键词：

D O I：

10.1016/0006-291X(79)91171-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DesGly10-DTrp6-LHRH Ethylamide, which possesses a 144 fold increased biological activity over native LHRH and is very resistant to proteolytic enzymes, was used as radioiodinated tracer, for the detection of LHRH receptor sites in preparations of ovine, bovine and rat pituitary plasma membranes. Up to 50% of [125I]-DesGly10DTrp6-LHRH-EA added could be bound by plasma membranes and displaced by LHRH analogs or native LHRH in relation to biological activity. Scatchard analysis showed only one class of binding sites (KA = 1010 M-1). The use of this LHRH analog as tracer offers a very convenient and precise method for the quantification of LHRH receptors in small amounts of tissues. © 1979.

引用

页码：1249 / 1256

页数：8

共 22 条

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