MAMMALIAN MESSENGER-RNAS ENCODING PROTEIN CLOSELY RELATED TO UBIQUITIN-CONJUGATING ENZYME ENCODED BY YEAST DNA-REPAIR GENE RAD6

被引：2

作者：

WOFFENDIN, C ^{[1
]}

CHEN, ZY ^{[1
]}

STASKUS, K ^{[1
]}

RETZEL, EF ^{[1
]}

PLAGEMANN, PGW ^{[1
]}

机构：

[1] UNIV MINNESOTA,SCH MED,DEPT MICROBIOL,MINNEAPOLIS,MN 55113

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1090卷 / 01期

关键词：

UBIQUITIN-CONJUGATING ENZYME MESSENGER RNA; YEAST RAD6 GENE; (HUMAN); (MOUSE); (RAT);

D O I：

10.1016/0167-4781(91)90039-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A clone of about 1 kb has been isolated from a human brain cDNA library. The clone possesses a 151 amino acid open reading frame that exhibits 72% amino acid identity with the E2 ubiquitin-conjugating enzyme encoded by the RAD6 gene of Saccharomyces cerevisiae. A 90% amino acid identity was observed in a central sequence surrounding a cysteine, which most likely contributes the sulfhydryl group involved in the formation of the ubiquitin-E2 thiolester linkage. Northern hybridization analyses have identified a poly(A)-containing mRNA of about 1 kb encoding the E2-like sequence in human CEM lymphoblastoid and HeLa cells, Novikoff rat hepatoma cells and S49 mouse leukemia cells. Southern hybridization analyses indicate the presence of a single gene encoding this sequence in both human cell lines, but of two or more related genes in the rodent cell lines.

引用

页码：81 / 85

页数：5

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