DETERMINATION OF THIAMINE BY CATHODIC STRIPPING VOLTAMMETRY

An electrochemical procedure for the determination of thiamine has been developed based on the cathodic electrogeneration of an electroactive product of thiamine (thiol form) on a mercury electrode, followed by cathodic constant potential electrolysis, constant potential oxidation and linear cathodic voltage-scan voltammetry. A linear calibration graph in the concentration range 1-100-mu-mol dm-3 of thiamine was observed. The reproducibility of the determination for 1 and 25-mu-mol dm-3 thiamine solutions, expressed in terms of the relative standard deviation (n = 7), was 8.8 and 6.6%, respectively.