THE SECONDARY STRUCTURE OF THE FERREDOXIN TRANSIT SEQUENCE IS MODULATED BY ITS INTERACTION WITH NEGATIVELY CHARGED LIPIDS

被引：33

作者：

HORNIAK, L

PILON, M

VANTHOF, R

DEKRUIJFF, B

机构：

[1] UNIV UTRECHT, DEPT MOLEC CELL BIOL, PADUALAAN 8, 3584 CH UTRECHT, NETHERLANDS

[2] COMENIUS UNIV BRATISLAVA, INST MOLEC & SUBCELLULAR BIOL, BRATISLAVA 81107, CZECHOSLOVAKIA

[3] UNIV UTRECHT, DEPT BIOCHEM MEMBRANES, CTR BIOMEMBRANES & LIPID ENZYMOL, 3584 CH UTRECHT, NETHERLANDS

来源：

FEBS LETTERS | 1993年 / 334卷 / 02期

关键词：

CHLOROPLAST PROTEIN IMPORT; TRANSIT PEPTIDE; CIRCULAR DICHROISM; PEPTIDE CONFORMATION; LIPID-PROTEIN INTERACTIONS;

D O I：

10.1016/0014-5793(93)81720-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Import of proteins into chloroplasts depends on an N-terminal transit sequence. Transit sequences contain little primary sequence similarity and therefore recognition of these sequences is thought to involve specific folding. To assess the conformational flexibility of the transit sequence, we studied the transit peptide of preferredoxin (trfd) by circular dichroism. In buffer, trfd is in a random coil conformation. A large increase in alpha-helix was induced in the presence of micelles or vesicles formed by anionic lipids. Less pronounced changes in secondary structure were induced by zwitterionic detergents but no changes were observed in the presence of neutral detergents or vesicles composed of phosphatidylcholine.

引用

页码：241 / 246

页数：6

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