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IDENTIFICATION OF FIBROBLAST GROWTH-FACTORS IN BOVINE CHEESE WHEY

被引：29
作者：
ROGERS, ML
BELFORD, DA
FRANCIS, GL
BALLARD, FJ
机构：
[1] Cooperative Research Centre for Tissue Growth and Repair, Adelaide, SA, 5000
来源：
JOURNAL OF DAIRY RESEARCH | 1995年 / 62卷 / 03期
关键词：
D O I：
10.1017/S0022029900031198
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin-sepharose column and eluted stepwise with 0.8, 1.2 and 2.0 M-NH4HCO3. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FQF in the 1.2 M-eluate and basic FGF in the 2.0 M-eluate, but neither acidic nor basic FGF was detected in the 0.8 M-fraction. Quantitative radioreceptor assays indicated 5.8 ng of acidic FGF-like activity and 19.8 ng of basic FGF-like activity per 1 whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.
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页码：501 / 507
页数：7
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