METABOLIC-REGULATION IN PLANT-CELL CULTURE .37. IDENTIFICATION OF NONEQUILIBRIUM GLYCOLYTIC REACTIONS IN SUSPENSION-CULTURED PLANT-CELLS

被引：34

作者：

KUBOTA, K ^{[1
]}

ASHIHARA, H ^{[1
]}

机构：

[1] OCHANOMIZU UNIV,FAC SCI,DEPT BIOL,2-1-1 OTSUKA,BUNKYO KU,TOKYO 112,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1036卷 / 02期

关键词：

(C. roseus); Enzyme kinetics; Glycolysis; Metabolic regulation; Plant cell culture;

D O I：

10.1016/0304-4165(90)90025-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Levels of all enzymes and metabolites involved in glycolysis were determined in suspension-cultured Catharanthus roseus cells. From both the maximum catalytic activities of the enzymes and comparisons of mass-action ratios with the apparent equilibrium constants for the reactions, it is concluded that the reactions catalysed by hexokinase, fructokinase, phosphofructokinase and pyruvate kinase are far from equilbrium, whereas the other reactions including that catalysed by pyrophosphate-fructose-6-phosphate 1-phosphotransferase, are close to equilibrium in vivo. © 1990.

引用

页码：138 / 142

页数：5

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