PURIFICATION AND CHARACTERIZATION OF PANCREATIC ELASTASE FROM ATLANTIC COD (GADUS-MORHUA)

被引：47

作者：

GILDBERG, A

OVERBO, K

机构：

[1] Institute of Fisheries Technology Research, N-9001 Tromsø

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 97卷 / 04期

关键词：

D O I：

10.1016/0305-0491(90)90122-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. A pancreatic elastase from Atlantic cod (Gadus morhua) has been purified and characterized. 2. The enzyme is a very basic protein with an approximate mol. wt of 28,000. 3. The cod elastase has higher elastin specificity than porcine elastase, and it is inhibited by soybean trypsin inhibitor, which has no effect on porcine elastase. 4. The cod elastase expresses a higher turnover number (k(cat)) and catalytic efficiency (k(cat)/K(m)) than porcine elastase, but it is less thermostable.

引用

页码：775 / 782

页数：8

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