EVALUATION OF A COMMERCIAL ANTI-DELTA EIA KIT FOR DETECTION OF ANTIBODIES TO HEPATITIS DELTA-VIRUS

被引：8

作者：

GOVINDARAJAN, S ^{[1
]}

VALINLUCK, B ^{[1
]}

LAKEBAKKAR, G ^{[1
]}

机构：

[1] SUNY STONY BROOK,STONY BROOK,NY 11794

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 1991年 / 95卷 / 02期

关键词：

D O I：

10.1093/ajcp/95.2.240

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Anti-hepatitis delta virus (anti-HDV) antibodies were measured by solid phase IgG and IgM capture radioimmunoassays (RIA) as well as by a competitive binding enzyme immunoassay (EIA) in both acute and chronic HDV infections. EIA anti-delta test measures total delta antibody without discriminating IgM from IgG anti-delta. Low titer IgG antibodies were detected by both techniques with equal sensitivity. High titer IgG antibodies reached the end point sooner with EIA than with RIA (10(-3) versus > 10(-6)). When IgM anti-HDV was present without accompanying IgG anti-HDV, EIA failed to identify the antibody. Presence of high titer rheumatoid factor in the serum and lipemic samples produced false-positive results by EIA. Usage of undiluted serum samples for EIA probably exaggerates the factors contributing to false-positive reaction.

引用

页码：240 / 241

页数：2