HUMAN SERUM-ALBUMIN INHIBITS PROSTACYCLIN PRODUCTION BY ENDOTHELIAL-CELLS - THE RELATION OF THE INHIBITORY ACTIVITY TO SULFHYDRYL-GROUPS IN ALBUMIN

被引：5

作者：

MUROHARA, Y ^{[1
]}

YUI, Y ^{[1
]}

HATTORI, R ^{[1
]}

KADOTA, K ^{[1
]}

KAWAI, C ^{[1
]}

机构：

[1] KYOTO UNIV,FAC MED,DEPT OBSTET & GYNECOL,DIV 3,KYOTO 606,JAPAN

来源：

JAPANESE CIRCULATION JOURNAL-ENGLISH EDITION | 1991年 / 55卷 / 08期

关键词：

ALBUMIN; PROSTAGLANDIN-I2; SULFHYDRYL COMPOUNDS;

D O I：

10.1253/jcj.55.777

中图分类号：

N09 [自然科学史]; B [哲学、宗教];

学科分类号：

01 ; 0101 ; 010108 ; 060207 ; 060305 ; 0712 ;

摘要：

Human serum inhibited calcium ionophore-induced production of prostacyclin by cultured bovine aortic endothelial cells. The inhibitory fraction was purified from serum by anion-exchange and Blue-Sepharose affinity chromatography. The molecular weight of the purified substance was 67k dalton as determined by sodium dodecyl sufate-polyacrylamide gel electrophoresis. It was identified as human serum albumin by N-terminal amino acid sequence analysis. Human serum albumin was separated to two forms by high-performance liquid chromatography: mercaptalbumin (SH type) and nonmercaptalbumin (SS type). Both types of albumin inhibited the conversion of arachidonic acid to prostaglandin H-2 in a dose-dependent manner without affecting phospholipase A2 or prostacyclin synthetase. This inhibition was more potent in mercaptalbumin than in nonmercaptalbumin. These results suggest that the conversion between mercaptalbumin and nonmercaptalbumin may play an important role in the modulation of prostacyclin synthesis by endothelial cells.

引用

页码：777 / 788

页数：12

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