EFFECTS OF INSULIN-LIKE GROWTH FACTOR-I ON INVITRO PRODUCTION OF BOVINE EMBRYOS

被引:82
作者
HERRLER, A [1 ]
LUCASHAHN, A [1 ]
NIEMANN, H [1 ]
机构
[1] INST TIERZUCHT & TIERVERHALTEN,NEUSTADT 1,GERMANY
关键词
INSULIN-LIKE GROWTH FACTOR-I; BOVINE; INVITRO MATURATION; INVITRO CULTURE; CUMULUS-OOCYTE COMPLEX EXPANSION;
D O I
10.1016/0093-691X(92)90177-S
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
A total of 917 cumulus-oocyte complexes were used in this experiment. In Group VI the cumulus-oocyte complexes were matured and fertilized in vitro. After an initial 48 hours culture in microdrops they were transferred to a granulosa cell monolayer. Development to morulae/blastocysts was evaluated 8 days after in-vitro fertilization. The treatment for Group I consisted of 50 ng/ml insulin-like growth factor I (IGF-I) added to in-vitro maturation media. Group II received IGF-I to in-vitro culture on the granulosa cell monolayer. In Group III of IGF-I was added to in-vitro maturation media and in-vitro culture on the granulosa cell monolayer. Group IV received IGF-I to in-vitro culture and was cultured the whole time without a granulosa cell monolayer. Group V received IGF-I to in-vitro maturation and were cultured in-vitro without a granulosa cell monolayer. After supplementation of in-vitro maturation media with IGF-I, 52, 49 and 51% of the cumulus-oocyte complexes in Groups I, III and V did not show cumulus expansion, whereas in Group VI (control) only 1% of the cumulus-oocyte complexes did not expand (P<0.01); in Groups II and IV 6 and 0%, respectively, did not expand. The cleavage rates in the five treatment groups were similar to those of the control group (55 to 71% vs 65%). In Group III, significantly more cleaved oocytes developed to morulae/blastocysts than in the control group (31 and 16%, respectively; P<0.05). In Groups IV and V only 2 and 5%, respectively, of the cleaved oocytes developed to morulae and blastocysts. These results demonstrate thatcumulus expansion does not permit the prediction of subsequent cleavage rates. Furthermore, addition of IGF-I to the in-vitro maturation and in-vitro culture media can increase the number of morulae and blastocysts, but addition of IGF-I could not replace co-culture with granulosa cells.
引用
收藏
页码:1213 / 1224
页数:12
相关论文
共 37 条
[1]   INSULIN-LIKE GROWTH-FACTORS AS INTRAOVARIAN REGULATORS OF GRANULOSA-CELL GROWTH AND FUNCTION [J].
ADASHI, EY ;
RESNICK, CE ;
DERCOLE, AJ ;
SVOBODA, ME ;
VANWYK, JJ .
ENDOCRINE REVIEWS, 1985, 6 (03) :400-420
[2]   SYNERGISTIC EFFECT OF INSULIN AND FOLLICLE-STIMULATING-HORMONE ON BIOCHEMICAL AND MORPHOLOGICAL-DIFFERENTIATION OF PORCINE GRANULOSA-CELLS INVITRO [J].
AMSTERDAM, A ;
MAY, JV ;
SCHOMBERG, DW .
BIOLOGY OF REPRODUCTION, 1988, 39 (02) :379-390
[3]  
BALBONI GC, 1987, FERTIL STERIL, V48, P796
[4]   FACTORS AFFECTING SUCCESSFUL INVITRO FERTILIZATION OF BOVINE FOLLICULAR OOCYTES [J].
BALL, GD ;
LEIBFRIED, ML ;
LENZ, RW ;
AX, RL ;
BAVISTER, BD ;
FIRST, NL .
BIOLOGY OF REPRODUCTION, 1983, 28 (03) :717-725
[5]  
BEDIRIAN KM, 1975, J ANIM SCI, V55, P251
[6]   FSH-INDUCED EXPANSION OF THE MOUSE CUMULUS-OOPHORUS INVITRO IS DEPENDENT UPON A SPECIFIC FACTOR(S) SECRETED BY THE OOCYTE [J].
BUCCIONE, R ;
VANDERHYDEN, BC ;
CARON, PJ ;
EPPIG, JJ .
DEVELOPMENTAL BIOLOGY, 1990, 138 (01) :16-25
[7]   AUTORADIOGRAPHIC DETECTION OF THE EARLIEST STAGE OF [H-3] URIDINE INCORPORATION INTO THE COW EMBRYO [J].
CAMOUS, S ;
KOPECNY, V ;
FLECHON, JE .
BIOLOGY OF THE CELL, 1986, 58 (03) :195-200
[8]   A STUDY OF THE 8-CELL TO 16-CELL DEVELOPMENTAL BLOCK IN BOVINE EMBRYOS CULTURED INVITRO [J].
EYESTONE, WH ;
FIRST, NL .
THERIOGENOLOGY, 1986, 25 (01) :152-152
[9]  
FUKUI Y, 1991, J REPROD FERTIL, V92, P125, DOI 10.1530/jrf.0.0920125
[10]  
GANDOLFI F, 1988, J REPROD FERTIL, V1, P63