CONTEMPORANEOUS NITROGEN-FIXATION AND DENITRIFICATION IN INTERTIDAL MICROBIAL MATS - RAPID RESPONSE TO RUNOFF EVENTS

We examined the contemporaneous responses of N2 fixation and denitrification to inorganic nitrogen-enriched runoff in 2 intertidal microbial mats in Tomales Bay (California, USA). Prior to runoff, N2 fixation rates averaged 3 mmol N m-2 d-1. Denitrification rates were lower, corresponding to 0 to 25 % of N2 fixation rates. After the initiation of runoff, N2 fixation rates decreased, approaching zero at both sites. In contrast, denitrification rates increased by an order of magnitude. We developed a simple model to examine the magnitude of N removal from creek water by mat denitrification before, during and after runoff. Model results show that during peak runoff, N removal was limited by the residence time of creek water in the intertidal region. As runoff volumes decreased and residence times increased, N removal became limited by the supply of organic matter. Our results illustrated the rapid metabolic adaptation of microbial mats to altered N fluxes. Changes occurring in the mat N cycling after the initiation of runoff led to 2 fundamental ecological changes: (1) mats became sinks for rather than sources of fixed N and (2) denitrification became limited by the availability of organic carbon rather than nitrate.