PHYSIOLOGICAL ACTIVITY OF WATER BEETLE DEFENSIVE AGENTS. II. ABSORPTION OF SELECTED ANESTHETIC STEROIDS AND NORSESQUITERPENES ACROSS GILL MEMBRANES OF THE MINNOW Pimephales promelas Raf.

The uptake of selected steroids and norsesquiterpenes by live minnows, Pimephales promelas, was studied when the compounds were administered externally in aqueous solution. The gills of minnows absorbed 80% of the steroid removed from solution. Steroid absorption across minnow gills is apparently a nonmediated process as rate saturation could not be demonstrated. Initial absorption rates of test compounds were inversely correlated with (1) the degree of oxygenation, (2) water solubility, and (3) polarity on silica gel thin-layer chromatography. These findings support the Stein model of nonmediated transport. The majority of compounds anesthetic and toxic to minnows exerted their effects at a similar internal concentration. Anesthesia occurred at ca. 10(-6)-10(-5) M and death at 10(-4)-10(-3) M. Since various agents causing membrane stabilization and lysis in in vitro systems act in an identical concentration range, it is proposed that the defensive steroids and norsesquiterpenes of water beetles act via membrane stabilization and lysis.