APPLICATION OF THE SOS UMU-TEST IN DETECTION OF POLLUTION USING FISH LIVER S9 FRACTION

被引:15
作者
BIHARI, N [1 ]
VUKMIROVIC, M [1 ]
BATEL, R [1 ]
ZAHN, RK [1 ]
机构
[1] ACAD SCI & LITERATURE MAINZ, COMMISS MOLEC BIOL, W-6500 MAINZ, GERMANY
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 1990年 / 95卷 / 01期
关键词
D O I
10.1016/0742-8413(90)90075-K
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. 1. The possibility of Aroclor 1254 and β-naphthoflavone treated fish Mugil auratus and fish sampled in low and high polluted areas to convert some premutagens to active intermediers in the SOS umu-test have been investigated. 2. 2. Genotoxicity of Aflatoxin b1 differed markedly upon activation with liver S9 fractions from fish with different pollution histories, with the highest activation potency in fish living near a fish cannery. 3. 3. Inhibition of umu gene expression by 7,8-benzoflavone in vitro clearly demonstrates a cytochrome P-450 mediated activation of aflatoxin b1. 4. 4. 2-Aminoanthracene and 2-aminofluorene were weakly activated to genotoxic products and the induction of umu gene expression could be detected only in the presence of S9 fractions from fish treated with β-naphthoflavone and Aroclor 1254 in the laboratory. 5. 5. The capability of S9 from fish living near a fish cannery to convert 2-aminoanthracene and 2-aminofluorene was not observed. © 1990.
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页码:15 / 18
页数:4
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