HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR ACID AND ALKALINE-PHOSPHATASE IN SERUM

被引:14
作者
KRSTULOVIC, AM [1 ]
HARTWICK, RA [1 ]
BROWN, PR [1 ]
机构
[1] UNIV RHODE ISL, DEPT CHEM, KINGSTON, RI 02881 USA
来源
JOURNAL OF CHROMATOGRAPHY | 1979年 / 163卷 / 01期
关键词
D O I
10.1016/S0378-4347(00)81164-0
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
High-performance liquid chromatography was used to assay serum acid and alkaline phosphatase. Samples were incubated with adenosine-5′-monophosphoric acid (AMP) in a buffer of required pH, 5′-nucleotidase was inhibited with Ni2+ ions, and the phosphatase activity was determined by measuring the concentration of the reaction product, adenosine. The analysis time, after the incubation is terminated, is short (7 min), and the assay is quantitative and reproducible. Complete separation of the reaction product from the substrate and the naturally occurring serum constituents and the high sensitivity of the ultra-violet detection system eliminate some of the problems commonly encountered in spectrophotometric assays. © 1979.
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页码:19 / 28
页数:10
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