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INTERACTION OF PUROMYCIN WITH ACCEPTOR SITE OF HUMAN PLACENTA 80-S RIBOSOMES

被引：4
作者：
GRAIFER, DM
FEDOROVA, OS
KARPOVA, GG
机构：
[1] Institute of Bioorganic Chemistry, Siberian Division, the USSR Academy of Sciences, Novosibirsk
来源：
FEBS LETTERS | 1990年 / 277卷 / 1-2期
关键词：
PUROMYCIN; RIBOSOME; HUMAN PLACENTA; RATE CONSTANT; AFFINITY;
D O I：
10.1016/0014-5793(90)80795-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The complex N-AcPhe-tRNA(Phe.)poly(U).80 S ribosome from human placenta was treated with puromycin taken in various concentrations. Based on the kinetic data of N-acetylphenylalanyl-puromycin formation, the association constant of puromycin with the acceptor site of the ribosome was estimated to be (3.96 +/- 0.84) x 10(4) M-1 at 37-degrees-C.
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页码：4 / 6
页数：3
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