CHEMOTHERAPY SCREENING ASSAY USING 3-DIMENSIONAL CELL-CULTURE

被引：5

作者：

KUPCHIK, HZ

COLLINS, EA

OBRIEN, MJ

MCCAFFREY, RP

机构：

[1] MALLORY INST PATHOL,BOSTON,MA 02118

[2] BOSTON UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02118

[3] BOSTON UNIV,SCH MED,DEPT MED,BOSTON,MA 02118

来源：

CANCER LETTERS | 1990年 / 51卷 / 01期

关键词：

3-D culture; alginate; chemosensitivity; colon carcinoma; organoid growth;

D O I：

10.1016/0304-3835(90)90224-L

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A new alginate culture method (ACM) was used to test the effects of vincristine and 5-fluorouracil (5-FU) on the commonly used human colon carcinoma cell line HT-29. Colorimetric analysis of viability following treatment with physiologically tolerated levels of vincristine showed significant reduction of the surviving cell population. Hematoxylin and eosin staining of sections of treated organoid growths corroborated the colorimetric analyses. The ACM is inexpensive, rapid and extremely versatile. Many alginate beads containing "mini-tissue" growths of many cell lines can be used simultaneously to evaluate numerous chemotherapeutic agents in an "in vivo-like" environment. © 1990.

引用

页码：11 / 16

页数：6

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