GENOME ANALYSIS OF IMPERFECT FUNGI - ELECTROPHORETIC KARYOTYPING AND CHARACTERIZATION OF THE NUCLEAR GENE CODING FOR GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GPD) OF CURVULARIA-LUNATA

被引：22

作者：

OSIEWACZ, HD

RIDDER, R

机构：

[1] Projektgruppe: Molekularbiologie der Alterungsprozesse, Deutsches Krebsforschungszentrum, Heidelberg, W-6900

来源：

CURRENT GENETICS | 1991年 / 20卷 / 1-2期

关键词：

CURVULARIA-LUNATA; PULSED-FIELD GEL ELECTROPHORESIS; ELECTROPHORETIC KARYOTYPE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GPD);

D O I：

10.1007/BF00312778

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) has been isolated from a genomic library of the filamentous fungus Curvularia lunata. The coding region of this gene consists of 1014 nucleotides and is interrupted by four introns. The gpd gene product shows a high degree of sequence identity with the corresponding proteins of various species belonging to both taxonomically related (e.g., Aspergillus nidulans), as well as more divergent, taxa. Using contour-clamped homogeneous electric field (CHEF) gel electrophoresis eight distinct chromosomal bands have been resolved, with two bands migrating as doublets and one as a triplet. Thus, the total number of chromosomes of C. lunata appears to be 12. The size of the chromosomes ranges from about 1.4 Mb to 4.0 Mb allowing an estimation of the genome to be approximately 29.7 Mb. By hybridization of fractionated chromosomes the gpd gene and the rDNA locus have been localized on individual chromosomes.

引用

页码：151 / 155

页数：5

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