REGULATION OF SYNTHESIS AND ACTIVITY OF NAD+-DEPENDENT 15-HYDROXY-PROSTAGLANDIN DEHYDROGENASE (15-PGDH) BY DEXAMETHASONE AND PHORBOL ESTER IN HUMAN ERYTHROLEUKEMIA (HEL) CELLS

被引：25

作者：

XUN, CQ ^{[1
]}

ENSOR, CM ^{[1
]}

TAI, HH ^{[1
]}

机构：

[1] UNIV KENTUCKY,COLL PHARM,DIV MED CHEM & PHARMACEUT,LEXINGTON,KY 40536

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90677-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dexamethasone stimulated 15-PGDH activity in HEL cells in a time and concentration dependent manner. Increase in 15-PGDH activity by dexamethasone was found to be accompanied by an increase in enzyme synthesis as revealedby Western blot and [35S]methionine labeling studies. In addition to dexamethasone, other anti-inflammatory steroids also increased 15-PGDH activity in the order of their glucocorticoid activity. Among sex steroids only progesterone increased significantly 15-PGDH activity. 12-0-Tetradecanoylphorbol-13-acetate (TPA) also induced the synthesis of 15-PGDH but inhibited the enzyme activity. It appears that TPA caused a time dependent inactivation of 15-PGDH by a protein kinase C mediated mechanism. © 1991.

引用

页码：1258 / 1265

页数：8

共 29 条

[1] PROGESTERONE AND ESTROGEN CONTROL OF UTERINE PROSTAGLANDIN DEHYDROGENASE-ACTIVITY DURING DECIDUOMAL GROWTH [J].

ALAM, NA ;

RUSSELL, PT ;

TABOR, MW ;

MOULTON, BC .

ENDOCRINOLOGY, 1976, 98 (04) :859-863

[2] PHORBOL ESTER INDUCIBLE GENES CONTAIN A COMMON CIS ELEMENT RECOGNIZED BY A TPA-MODULATED TRANS-ACTING FACTOR [J].

ANGEL, P ;

IMAGAWA, M ;

CHIU, R ;

STEIN, B ;

IMBRA, RJ ;

RAHMSDORF, HJ ;

JONAT, C ;

HERRLICH, P ;

KARIN, M .

CELL, 1987, 49 (06) :729-739

[3] BIOLOGICAL ACTIVITIES OF 3 METABOLITES OF PROSTAGLANDIN E1 [J].

ANGGARD, E .

ACTA PHYSIOLOGICA SCANDINAVICA, 1966, 66 (04) :509-&

[4]

ANGGARD E, 1964, J BIOL CHEM, V239, P4097

[5]

BEDWAIN JR, 1978, BRIT J PHARMACOL, V53, P547

[6]

BLACKWELL GJ, 1976, BRIT J PHARMACOL, V56, pP343

[7]

CASEY ML, 1988, J BIOL CHEM, V263, P7846

[8]

ENSOR CM, 1990, J BIOL CHEM, V265, P14888

[9] 11TH GADDUM MEMORIAL LECTURE - LIPOCORTIN AND THE MECHANISM OF ACTION OF THE GLUCOCORTICOIDS [J].

FLOWER, RJ .

BRITISH JOURNAL OF PHARMACOLOGY, 1988, 94 (04) :987-1015

[10]

FU JY, 1990, J BIOL CHEM, V265, P16737

← 1 2 3 →