CLONING AND SEQUENCING OF A PCR AMPLIFIED GAMMA-GLIADIN GENE FROM DURUM-WHEAT (TRITICUM-TURGIDUM (L) THELL CONV DURUM (DESF) MK)

被引：22

作者：

DOVIDIO, R

TANZARELLA, OA

PORCEDDU, E

机构：

[1] Dipartimento di Agrobiologia e Agrochimica, Universitá della Tuscia, 01100 Viterbo, via S. Camillo de Lellis

来源：

PLANT SCIENCE | 1991年 / 75卷 / 02期

关键词：

WHEAT STORAGE PROTEINS; PCR; GENE CLONING; TRITICUM-TURGIDUM; DURUM WHEAT;

D O I：

10.1016/0168-9452(91)90238-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific amplification by PCR of gamma-gliadin genes from Triticum turgidum conv. durum cv. Langdon was obtained using high temperature for DNA-primers annealing. Three amplified bands were obtained and one of these was cloned and sequenced. Comparison of this nucleotide sequence with those of published gamma-gliadin and gamma-hordein genes revealed a high sequence homology. In particular, the comparison with pW1020 clone isolated from T. aestivum showed that the sequences derived from two genotypes differed by only two nucleotides. The advantages offers by this approach for rapid gene isolation from different genotypes and its importance in molecular genetic analysis of wheat storage proteins are discussed.

引用

页码：229 / 236

页数：8

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