DEVELOPMENT OF AN OROFACIAL MODEL OF ACUTE-INFLAMMATION IN THE RAT

被引：77

作者：

HAAS, DA ^{[1
]}

NAKANISHI, O ^{[1
]}

MACMILLAN, RE ^{[1
]}

JORDAN, RC ^{[1
]}

HU, JW ^{[1
]}

机构：

[1] KYUSHU DENT SCH,KITAKYUSHU,FUKUOKA 803,JAPAN

来源：

ARCHIVES OF ORAL BIOLOGY | 1992年 / 37卷 / 05期

关键词：

INFLAMMATION; TEMPOROMANDIBULAR JOINT; MUSTARD OIL; PLASMA PROTEIN EXTRAVASATION; POLYMORPHONUCLEAR NEUTROPHILS;

D O I：

10.1016/0003-9969(92)90026-5

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

An appropriate model was created by the paraperiosteal injection of mustard oil (20% ally] isothiocyanate dissolved in mineral oil) into the periarticular temporomandibular tissue of anaesthetized rats. Inflammation was assessed by the plasma extravasation of Evans' blue dye bound to plasma protein. This was confirmed visually and compared spectrophotometrically with the contralateral untreated control site (p < 0.0005). A time-course study of the effect of mustard oil on Evans' blue extravasation revealed a gradually increasing effect that was maximal at 30 min after administration, with no further increase at 60 min. A dose-response study showed that giving 30-mu-l of 20% mustard oil produced the maximal effect, with no further increase from 50-mu-l. To confirm induction of inflammation, polymorphonuclear neutrophil infiltration was assessed morphometrically and found to increase in the treated tissue compared with the contralateral untreated control (p < 0.001),

引用

页码：417 / 422

页数：6

共 22 条

[1] OPIATE AGONISTS INHIBIT NEUROGENIC PLASMA EXTRAVASATION IN THE RAT [J].