PARTIAL-PURIFICATION AND PROPERTIES OF A NOVEL CHITOSANASE SECRETED BY RHODOTORULA-GRACILIS

被引:17
作者
SOMASHEKAR, D [1 ]
JOSEPH, R [1 ]
机构
[1] CENT FOOD TECHNOL RES INST,MYSORE 570013,KARNATAKA,INDIA
关键词
D O I
10.1111/j.1472-765X.1992.tb00633.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An extracellular chitosanase produced by Rhodotorula gracilis CFR-1 that catalyses a limited degradation of chitosan with no detectable generation of glucosamine or reducing groups was identified. Ultracentrifugation, polyacrylamide gel electrophoresis and gel permeation studies suggest that chitosan of average molecular mass 36 000 Da was reduced by the enzymic catalysis to nearly one-fourth this size without further hydrolysis of the products. The enzyme, produced constitutively by this yeast, was partially purified and some of its properties were studied.
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页码:1 / 4
页数:4
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