MECHANISM OF AMMONIUM TRANSPORT BY INTESTINAL SEGMENTS FOLLOWING URINARY-DIVERSION - EVIDENCE FOR IONIZED NH4+ TRANSPORT VIA K+-PATHWAYS

被引:25
作者
HALL, MC
KOCH, MO
MCDOUGAL, WS
机构
[1] VANDERBILT UNIV,MED CTR,SCH MED,DEPT UROL,MED CTR N,NASHVILLE,TN 37232
[2] MASSACHUSETTS GEN HOSP,DEPT UROL,BOSTON,MA 02114
关键词
URINARY DIVERSION; AMMONIA; POTASSIUM;
D O I
10.1016/S0022-5347(17)36627-2
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Using a previously reported in vivo intestinal perfusion model in the rat, we have shown that net total ammonium absorption accounts for the majority of the acid load resulting from urinary intestinal diversion. In the present study, by varying perfusate pH and therefore NH3 concentrations, we demonstrated that the net flux of total ammonium did not correlate with non-ionized NH3 concentrations (r = .039). This indicates that the basic mechanism of total ammonium flux is via ionized NH4+ movement. To more precisely define the transport processes involved, we manipulated this system with the following chemical and pharmacologic probes of electrolyte transport: amiloride (0.5 mM/l.), furosemide (1 mM/l.), 2,4,6-triaminopyrimidine (TAP) (15 mM/l.), methylprednisilone (3 mg./100gm./B.W.) S.Q. x 3 days followed by perfusion, and barium (Ba2+) (15 mM/l.). Net solute flux was not significantly altered by the mucosal addition of amiloride or TAP. Methylprednisilone treated rats exhibited significantly diminished Na+ secretion (p < .01) and increased Cl- absorption (p <.05) without affecting net total ammonium flux providing evidence for the inducibility of Na+ conductance channels and against significant NH4+ movement via this pathway. The mucosal addition of furosemide resulted in significantly decreased net absorption of both total ammonium (p <.001) and K+ (p <.05). The addition of Ba2+ resulted in a three-fold reduction of ammonium absorption (p <.001) and a greater than ten-fold reduction in K+ absorption (p <.001). The observation of significant inhibition of ammonium absorption by furosemide and barium suggests that K+ transport pathways play a significant role in the intestinal transport of NH4+.
引用
收藏
页码:453 / 457
页数:5
相关论文
共 18 条
[1]   AMILORIDE - A MOLECULAR PROBE OF SODIUM-TRANSPORT IN TISSUES AND CELLS [J].
BENOS, DJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (03) :C131-C145
[2]  
BROWN RL, 1975, CLIN SCI MOL MED, V48, P179
[3]  
CASTELL DO, 1971, GASTROENTEROLOGY, V60, P33
[4]  
COHEN RM, 1988, AM J PHYSIOL, V23, pF657
[5]  
DONOWITZ M, 1987, PHYSL GASTROINTESTIN, P1351
[6]  
GARVIN JL, 1987, AM J PHYSIOL, V249, pF232
[7]   ACTIVE ABSORPTION OF NH4+ BY RAT MEDULLARY THICK ASCENDING LIMB - INHIBITION BY POTASSIUM [J].
GOOD, DW .
AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (01) :F78-F87
[8]   AMMONIUM TRANSPORT IN MEDULLARY THICK ASCENDING LIMB OF RABBIT KIDNEY - INVOLVEMENT OF THE NA(+)-COTRANSPORTER,K(+)-COTRANSPORTER,CL(-)-COTRANSPORTER [J].
KINNE, R ;
KINNESAFFRAN, E ;
SCHUTZ, H ;
SCHOLERMANN, B .
JOURNAL OF MEMBRANE BIOLOGY, 1986, 94 (03) :279-284
[9]  
KINNE R, 1985, PFLUEGERS ARCH, V405, P5101
[10]   AMMONIUM TRANSPORT IN THE KIDNEY [J].
KNEPPER, MA ;
PACKER, R ;
GOOD, DW .
PHYSIOLOGICAL REVIEWS, 1989, 69 (01) :179-249