A RAPID ATTENUATION OF MUSCARINIC AGONIST-STIMULATED PHOSPHOINOSITIDE HYDROLYSIS PRECEDES RECEPTOR SEQUESTRATION IN HUMAN SH-SY-5Y NEUROBLASTOMA-CELLS

被引：23

作者：

FISHER, SK ^{[1
]}

SLOWIEJKO, DM ^{[1
]}

MCEWEN, EL ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT PHARMACOL,ANN ARBOR,MI 48104

来源：

NEUROCHEMICAL RESEARCH | 1994年 / 19卷 / 05期

关键词：

MUSCARINIC CHOLINERGIC RECEPTOR; PHOSPHOINOSITIDE HYDROLYSIS; INOSITOL 1,4,5-TRISPHOSPHATE; SH-SY-5Y NEUROBLASTOMA; DESENSITIZATION;

D O I：

10.1007/BF00971329

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Agonist occupancy of muscarinic cholinergic receptors in human SH-SY-5Y neuroblastoma cells elicited two kinetically distinct phases of phosphoinositide hydrolysis when monitored by either an increased mass of inositol 1,4,5-trisphosphate, or the accumulation of a total inositol phosphate fraction. Within 5s of the addition of the muscarinic agonist, oxotremorine-M, the phosphoinositide pool was hydrolyzed at a maximal rate of 9.5%/min. This initial phase of phosphoinositide hydrolysis was short-lived (t(1/2) = 14s) and after 60s of agonist exposure, the rate of inositol lipid breakdown had declined to a steady stare level of 3.4%/min which was then maintained for at least 5-10 min. This rapid, but partial, attenuation of muscarinic receptor stimulated phosphoinositide hydrolysis occurred prior to the agonist-induced internalization of muscarinic receptors.

引用

页码：549 / 554

页数：6

共 22 条

[1] REDUCTION OF MUSCARINIC RECEPTOR DENSITY AND OF GUANINE NUCLEOTIDE-STIMULATED PHOSPHOINOSITIDE HYDROLYSIS IN HUMAN SH-SY5Y NEUROBLASTOMA-CELLS FOLLOWING LONG-TERM TREATMENT WITH 12-O-TETRADECANOYLPHORBOL 13-ACETATE OR MEZEREIN [J].