ACTIN FILAMENT ANNEALING IN THE PRESENCE OF ATP AND PHALLOIDIN

被引：32

作者：

KINOSIAN, HJ

SELDEN, LA

ESTES, JE

GERSHMAN, LC

机构：

[1] DEPT VET AFFAIRS SAMUEL S STRATTON MED CTR, RES SERV 151-B, HOLLAND AVE, ALBANY, NY 12208 USA

[2] ALBANY MED COLL, DEPT PHYSIOL & BIOPHYS, ALBANY, NY 12208 USA

[3] ALBANY MED COLL, DEPT MED, ALBANY, NY 12208 USA

[4] DEPT VET AFFAIRS SAMUEL S STRATTON MED CTR, MED SERV, ALBANY, NY 12208 USA

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 46期

关键词：

D O I：

10.1021/bi00097a011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The re-formation of actin filaments after fragmentation by sonication in the presence of phalloidin and ATP has been found to follow second-order kinetics. The data are described by a model in which the rate of actin filament annealing is proportional to the square of the number concentration of actin filaments and the rate of fragmentation is proportional to the actin polymer concentration. In the presence of 100 mM KCl, 1 mM MgCl2, and equimolar phalloidin, the second-order rate constant for annealing of actin filaments is 2.2 X 10(6) M-1 s-1 and the first-order rate constant for fragmentation is 7 X 10(-7) s-1. In addition, the observed pseudo-first-order rate constant for annealing was found to increase with increasing ionic strength. Thus, annealing may play a major part in the length redistribution phase of actin polymerization and may be important for actin filament rearrangement in the cell.

引用

页码：12353 / 12357

页数：5

共 25 条

[1] DISTRIBUTION OF ACTIN FILAMENT LENGTHS MEASURED BY FLUORESCENCE MICROSCOPY [J].