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MICROPROPAGATION OF MATURE SIBERIAN ELM IN 2 STEPS

被引：14
作者：
CHENG, ZM
SHI, NQ
机构：
[1] Department of Plant Sciences, North Dakota State University, Fargo, 58105, ND
[2] Department of Genetics, University of Wisconsin-Madison, Madison, 53706, WI
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1995年 / 41卷 / 02期
关键词：
BENZYLADENINE; GELLING AGENT; MICROPROPAGATION; ULMUS PUMILA;
D O I：
10.1007/BF00051592
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A simple and reliable two-step micropropagation system was developed for mature Siberian elm (Ulmus pumila L.). Shoot-tip cultures were initiated and multiplied on a modified Murashige and Skoog medium supplemented with 1 mu M benzyladenine, B5 vitamins, solidified with 0.22% Gelrite, and subcultured weekly. Proliferated shoots 2-3 cm in length rooted successfully in sterile and non-sterile soil mix at 100% efficiency. Healthy plants were acclimatized to the ambient environment.
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页码：197 / 199
页数：3
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