ON COMPOSITION OF NUCLEOLUS WITH SPECIAL REFERENCE TO ITS FILAMENTOUS STRUCTURE

Different staining procedures, various digestion methods and autoradiographic techniques were employed to study the structure and composition of the nucleolus and of the nucleolonema, after unmasking the latter by adenosine treatment. The presence of DNA, RNA, protein and lipid in these structures has been shown. It has been demonstrated that the filamentous structure within the nucleolus - the nucleolonema- has a core of DNA, around which RNA and protein have accumulated. The structure of the nucleolonema suggests that it is in a highly active state, in synthesizing ribosomal RNA and protein. © 1969 Springer-Verlag.