GAS-CHROMATOGRAPHY AND MASS-SPECTROMETRY OF DISACCHARIDES FROM GLYCOPROTEINS

被引：1

作者：

BUSH, CA

机构：

[1] Department of Chemistry, Illinois Institute of Technology, Chicago

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 04期

关键词：

gas chromatography; glycoproteins; hydrolysis; mass spectrometry; methanolysis; silylation;

D O I：

10.1016/0165-022X(79)90009-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Partial acid hydrolysis and methanolysis released disaccharides and disaccharide methylglycosides from the glycoproteins, ovomucoid and porcine gastric mucin in amounts of 0.5-7 μg disaccharide per mg of glycoprotein. These disaccharides were fractionated by gas chromatography as the trimethylsilyl (Me3Si) derivatives. The composition of recovered disaccharides has been determined by hydrolisis and rechromatography of the Me3Si monosaccharides. The intersaccharide linkages of the disaccharides have been determined by electron impact mass spectrometry. This simple and rapid method can give structural information on small glycoprotein samples. © 1979.

引用

页码：227 / 235

页数：9

共 10 条

[1] ANALYTICAL AND COMPARATIVE CHROMATOGRAPHIC MAPS OF OLIGOSACCHARIDES PRODUCED BY ACETOLYSIS AND PARTIAL ACID-HYDROLYSIS OF GLYCOPROTIDS [J].