BIOLOGICAL ROLE OF RIBOSOMAL-PROTEIN BM-L11 OF BACILLUS-MEGATERIUM, HOMOLOGOUS WITH ESCHERICHIA-COLI RIBOSOMAL-PROTEIN L11

Ribosomes from a thiostrepton-resistant mutant of Bacillus megaterium lack a protein, BM-L11, which is homologous with Escherichia coli ribosomal protein L11. Such ribosomes retain partial activity in cell-free synthesis of polyphenylalanine and can be restored to full activity by reconstitution with protein BM-L11. Examination of individual steps involved in polypeptide chain elongation suggested a role for protein BM-L11, and by inference for E. coli protein L11, in promoting the ribosomal GTP hydrolysis dependent upon elongation factor EF G. Evidently, however, protein BM-L11 is not indispensable for ribosomal function. © 1979.