CHARACTERIZATION OF A MEDIUM WAVELENGTH TYPE DNA PHOTOLYASE - PURIFICATION AND PROPERTIES OF PHOTOLYASE FROM BACILLUS-FIRMUS

被引：42

作者：

MALHOTRA, K ^{[1
]}

KIM, ST ^{[1
]}

SANCAR, A ^{[1
]}

机构：

[1] UNIV N CAROLINA, SCH MED, DEPT BIOCHEM & BIOPHYS, CHAPEL HILL, NC 27599 USA

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 29期

关键词：

D O I：

10.1021/bi00195a012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene for the apoenzyme of Bacillus firmus photolyase was cloned and sequenced. The enzyme was overproduced in Escherichia coli, purified, and characterized. It has the unique property of having the maximum activity over a wavelength range where all other known photolyases exhibit modest activity. The enzyme contains reduced FAD and methenyltetrahydrofolate and has an absorption and action spectrum peak at 410 nm, and it repairs DNA with a quantum yield of phi similar to 0.75.

引用

页码：8712 / 8718

页数：7

共 21 条

[1]

EKER APM, 1990, J BIOL CHEM, V265, P8009

[2]

HAMMALVAREZ S, 1990, J BIOL CHEM, V265, P18656

[3] PHOTOREACTIVATION OF KILLING IN STREPTOMYCES - ACTION SPECTRA AND KINETIC STUDIES [J].

JAGGER, J ;

TAKEBE, H ;

SNOW, JM .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1970, 12 (03) :185-&

[4] IDENTIFICATION OF THE 2ND CHROMOPHORE OF ESCHERICHIA-COLI AND YEAST DNA PHOTOLYASES AS 5,10-METHENYLTETRAHYDROFOLATE [J].

JOHNSON, JL ;

HAMMALVAREZ, S ;

PAYNE, G ;

SANCAR, GB ;

RAJAGOPALAN, KV ;

SANCAR, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (07) :2046-2050

[5] CHROMOPHORE FUNCTION AND INTERACTION IN ESCHERICHIA-COLI DNA PHOTOLYASE - RECONSTITUTION OF THE APOENZYME WITH PTERIN AND OR FLAVIN DERIVATIVES [J].

JORNS, MS ;

WANG, BY ;

JORDAN, SP ;

CHANDERKAR, LP .

BIOCHEMISTRY, 1990, 29 (02) :552-561

[6] DETERMINATION OF RATES AND YIELDS OF INTERCHROMOPHORE (FOLATE-]FLAVIN) ENERGY-TRANSFER AND INTERMOLECULAR (FLAVIN-]DNA) ELECTRON-TRANSFER IN ESCHERICHIA-COLI PHOTOLYASE BY TIME-RESOLVED FLUORESCENCE AND ABSORPTION-SPECTROSCOPY [J].

KIM, ST ;

HEELIS, PF ;

OKAMURA, T ;

HIRATA, Y ;

MATAGA, N ;

SANCAR, A .

BIOCHEMISTRY, 1991, 30 (47) :11262-11270

[7] EFFECT OF BASE, PENTOSE, AND PHOSPHODIESTER BACKBONE STRUCTURES ON BINDING AND REPAIR OF PYRIMIDINE DIMERS BY ESCHERICHIA-COLI DNA PHOTOLYASE [J].

KIM, ST ;

SANCAR, A .

BIOCHEMISTRY, 1991, 30 (35) :8623-8630

[8] MOLECULAR CHARACTERIZATION OF A GENE ENCODING A PHOTOLYASE FROM STREPTOMYCES-GRISEUS [J].

KOBAYASHI, T ;

TAKAO, M ;

OIKAWA, A ;

YASUI, A .

NUCLEIC ACIDS RESEARCH, 1989, 17 (12) :4731-4744

[9]

MALHOTRA K, 1992, J BIOL CHEM, V267, P15406

[10]

MALHOTRA K, 1992, J BIOL CHEM, V267, P2909

← 1 2 3 →