SPIN-LABELED HAPTEN STUDIES OF STRUCTURE HETEROGENEITY AND CROSS-REACTIVITY OF ANTIBODY ACTIVE SITE

被引：15

作者：

HSIA, JC

PIETTE, LH

机构：

[1] Department of Biochemistry and Biophysics, School of Medicine, University of Hawaii, Honolulu

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 132卷 / 02期

关键词：

D O I：

10.1016/0003-9861(69)90390-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ESR spin-labeling is used to probe the structural heterogeneity of rabbit anti-DNP antibodies. Antibodies isolated with homologous and cross reacting antigens yield different degrees of immobilization when bound with a homologous spin-labeled hapten. Different degrees of immobilization are observed between cross reacting haptens and homologous haptens. These differences in spin-label immobilization are discussed in terms of structural heterogeneity of the combining site. © 1969.

引用

页码：466 / &

共 9 条

[1] CHENG WC, 1965, J BIOL CHEM, V240, P3530
[2] THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP
FARAH, FS
KERN, M
EISEN, HN
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1960, 112 (06) : 1195 - 1210
[3] NITROXIDE FREE RADICALS - SPIN LABELS FOR PROBING BIOMOLECULAR STRUCTURE
GRIFFITH, OH
WAGGONER, AS
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1969, 2 (01) : 17 - &
[4] HAMILTON CL, 1968, STRUCTURAL CHEMISTRY, P115
[5] SPIN-LABELING AS A GENERAL METHOD IN STUDYING ANTIBODY ACTIVE SITE
HSIA, JC
PIETTE, LH
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1969, 129 (01) : 296 - &
[6] KOSMAN DJ, IN PRESS
[7] EVIDENCE FOR TRYPTOPHAN IN ACTIVE SITES OF ANTIBODIES TO POLYNITROBENZENES
LITTLE, JR
EISEN, HN
[J]. BIOCHEMISTRY, 1967, 6 (10) : 3119 - &
[8] FREE IMINOXYL RADICALS IN HYDROGENATED PYRROLE SERIES
ROZANTZE.EG
KRINITZK.LA
[J]. TETRAHEDRON, 1965, 21 (03) : 491 - &
[9] EXCITATION ENERGY TRANSFER AND THE QUANTITATIVE STUDY OF THE ANTIBODY HAPTEN REACTION
VELICK, SF
PARKER, CW
EISEN, HN
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1960, 46 (11) : 1470 - 1482

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