DEVELOPMENT OF A PIEZOELECTRIC IMMUNOSENSOR FOR THE DETECTION OF SALMONELLA-TYPHIMURIUM

被引：110

作者：

PRUSAKSOCHACZEWSKI, E ^{[1
]}

LUONG, JHT ^{[1
]}

GUILBAULT, GG ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,6100 ROYAL MT AVE,MONTREAL H4P 2R2,QUEBEC,CANADA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 03期

关键词：

antibody; immunosensor; Piezoelectric crystal; Salmonella;

D O I：

10.1016/0141-0229(90)90034-N

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A piezoelectric biosensor has been developed for the detection of Salmonella typhimurium. The antibody to Salmonella was immobilized on the crystal by various immobilization procedures. The best result was obtained when antibody was immobilized on the crystal precoated with a thin layer of polyethyleneimine. The response of the coated crystal for S. typhimurium in a microbial suspension was in the range of 105 to 109 cells ml-1. The time required for a complete interaction between the crystal and the cells appeared to depend upon the cell concentration of the analyzed sample. The antibody-bound crystal lost no activity over 4 days at 4°C and it could be reused for 6-8 consecutive assays. © 1990.

引用

页码：173 / 177

页数：5

共 15 条

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