PURIFICATION AND PROPERTIES OF 2'-NUCLEOTIDASE FROM MAMMALIAN BRAIN

被引：12

作者：

NAKAMURA, S

YAMAO, S

ITO, J

KAMEYAMA, M

机构：

[1] Department of Geriatrics, Kyoto University Hospital, Sakyo-ku, Kyoto

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 568卷 / 01期

关键词：

(Mammalian brain); 2′-Nucleotidase; Glycerol; 2-phosphate;

D O I：

10.1016/0005-2744(79)90270-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A nucleotidase specific for 2′-nucleotides was localized in both the soluble and the synaptosomal fractions of rat brain. The enzyme was partially purified from the soluble fraction of bovine brain. The 220,w was 4.9 S with an estimated molecular weight of about 70 000. The optimum pH was 8.0 and Km value for 2′-AMP was 5.5 · 10-4 M. The substrate and inhibitor specificities of the enyzme were examined. The nucleotidase has an absolute requirement for Mg2+ but neither Fe3+ nor Ca2+ acted as replacement ions. In fact, Mn2+ and Ca2+ inhibited the Mg2+-dependent 2′-nucleotidase. © 1979.

引用

页码：30 / 38

页数：9

共 24 条

[1]

ANRAKU Y, 1964, J BIOL CHEM, V239, P3412

[2]

ARSENIS C, 1968, J BIOL CHEM, V243, P5702

[3] METHODS OF ISOLATION AND CHARACTERIZATION OF MONONUCLEOTIDES AND POLYNUCLEOTIDES BY ION EXCHANGE CHROMATOGRAPHY [J].