DETERMINATION OF AMIKACIN ISOMERS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引：10

作者：

MAITRA, SK ^{[1
]}

YOSHIKAWA, TT ^{[1
]}

STEYN, CM ^{[1
]}

GUZE, LB ^{[1
]}

SCHOTZ, MC ^{[1
]}

机构：

[1] HARBOR GEN HOSP,DEPT MED,TORRANCE,CA 90509

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1979年 / 2卷 / 06期

关键词：

D O I：

10.1080/01483917908060106

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-pressure liquid chromatographic (HPLC) method for quantitative monitoring of amikacin isomers is described. Four isomers, BB-K8, BB-K29, BB-K6 and BB-K11 were applied to a silica gel column. While adsorbed, the isomers were derivatized with o-phthalaldehyde and the derivatized products eluted with ethanol. A decrease in the fluorescence of the derivatized products with time was observed. Heating at 50°C for 5 min produced products with stable fluorescence for at least three hours. Using the fluorescent properties of the amikacin derivative for detection, the four isomers of amikacin were separated by reverse phase (HPLC). A linear relationship from 1 to 10 μg/mL was obtained for all four isomers. Copyright © 1979, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：823 / 836

页数：14

共 7 条

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JOURNAL OF ANTIBIOTICS, 1974, 27 (11) :851-858

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