EFFICIENT ELECTROTRANSFORMATION OF ENTEROCOCCUS-HIRAE WITH A NEW ENTEROCOCCUS ESCHERICHIA-COLI SHUTTLE VECTOR

被引：24

作者：

SOLIOZ, M

WASER, M

机构：

[1] Dept of Clinical Pharmacology, University of Berne, 3010 Berne

来源：

BIOCHIMIE | 1990年 / 72卷 / 04期

关键词：

electroporation; Enterococcus; improved electroporation unit; shuttle-vector; transformation;

D O I：

10.1016/0300-9084(90)90084-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, an Enterococcus-Echerichia coli shuttle vector, pC3, was constructed that allows efficient transformation by electroporation of Enterococcus hirae ATCC9790. 5 × 106 transformants per microgram of plasmid DNA were obtained, using a commercial capacitor discharge device with an improved circuitry and a home-made electrode assembly, delivering pulses of 24 kV/cm across the cell suspension. The transformants were stable without selective pressure and plasmid DNA reisolated from transformed cells displayed no alterations in restriction enzyme analysis. Chromosomal DNA from E coli or E hirae, carried by pC3, was stably maintained in E hirae, making cloning and genetic manipulation in this organism feasible. © 1990.

引用

页码：279 / 283

页数：5

共 32 条

[1] SIMPLE AND RAPID METHOD FOR ISOLATING LARGE PLASMID DNA FROM LACTIC STREPTOCOCCI [J].