FLOW-CYTOMETRY AS A TOOL TO DISCRIMINATE RESPIRATORY-COMPETENT AND RESPIRATORY-DEFICIENT YEAST-CELLS

被引：39

作者：

SKOWRONEK, P ^{[1
]}

KRUMMECK, G ^{[1
]}

HAFERKAMP, O ^{[1
]}

RODEL, G ^{[1
]}

机构：

[1] LAB MOLEK BIOL & ALLGEMEINE PATHOL,W-8000 MUNICH 45,GERMANY

来源：

CURRENT GENETICS | 1990年 / 18卷 / 03期

关键词：

Flow cytometry; Respiratory chain; Rhodamine; 123; Yeast;

D O I：

10.1007/BF00318391

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The cationic lipophilic dye Rhodamine 123 (Rh123) is selectively enriched in mitochondria in a membrane potential-dependent manner. Application of drugs which interfere with the electron flow of the respiratory chain lead to a severe reduction of mitochondrial dye uptake. In this communication we show that the same effect is observed after Rh123-staining of respiratory-deficient yeast mutants. Based on this observation we used flow cytometry to discriminate respiratory-compentent and respiratory-deficient yeast cells. Combined with a cell sorter we were able to selectively enrich respiring and non-respiring yeast cells, repectively, from a mixture of cells. © 1990 Springer-Verlag.

引用

页码：265 / 267

页数：3

共 10 条

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