METHODS FOR SEQUENCING OLIGORIBONUCLEOTIDES AND RNA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：5

作者：

KOMIYA, H

NISHIKAWA, K

OGAWA, K

TAKEMURA, S

机构：

[1] Institute of Molecular Biology, School of Science, Nagoya University, Nagoya, Aichi 464, Chikusa-ku

来源：

JOURNAL OF BIOCHEMISTRY | 1979年 / 86卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a132602

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conditions were established for the separation and quantitative determination of ribonucleo-sides, mono- and oligo-ribonucleotides by high-performance liquid chromatography (HPLC) on columns of AS-Pellionex SAX and AL-Pellionex WAX. By combining a high-speed UV spectrum monitor with an HPLC apparatus, products of RNase digestions of oligo-nucleotides and 5S ribosomal RNA (rRNA) were identified by measuring their UV spectra under continuous solvent flow, and also from their retention times on the columns (positions of elution). It took only 10 to 30 min for one chromatography run and required less than 0.01 A260 unit of sample per nucleotide material in each peak. © 1979, by the Japanese Biochemical Society.

引用

页码：1081 / 1088

页数：8

共 16 条

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