TROPHOBLAST TISSUE-CULTURE OF HUMAN INTRAUTERINE AND ECTOPIC PREGNANCIES AND TREATMENT WITH METHOTREXATE

被引：17

作者：

SCHAFER, D

PFUHL, JP

BAUMANN, R

NEUBERT, S

BENDER, HG

NAUJOKS, H

机构：

[1] Department of Obstetrics/Gynaecology, ZFG, Universitätsklinikum Frankfurt/Main, D-6000 Frankfurt/Main 70

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 03期

关键词：

ECTOPIC; CULTURE; HCG; METHOTREXATE; TROPHOBLAST;

D O I：

10.1093/oxfordjournals.humrep.a137641

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The application of drugs for conservative treatment of patients with ectopic pregnancy has been used worldwide for several years. In-vitro studies, however, are verv few. We therefore examined the effects of methotrexate on trophoblast tissue cultures derived from intrauterine and ectopic pregnancies. Methotrexate was administered either 12 h or 6 days after initiation of the culture. Human chorionic gonadotrophin (HCG) levels were measured in the culture medium. All cultures showed secretion of HCG within the first 16 days. Methotrexate concentrations < 3.8 x 10(-4) mol/l had no effect on HCG secretion. Cultures of ectopic pregnancies required a concentration about 10 x higher to induce an equivalent reduction of HCG levels compared to intrauterine pregnancies. A few intrauterine and ectopic pregnancies showed no reduction of HCG values after treatment. These results suggest that data obtained from studies on intrauterine pregnancies may not be transferable to ectopic pregnancies in all instances. In some regimens approximately 8 days are required before the effect becomes measurable. In combination with our clinical data, we therefore recommend not to repeat a methotrexate dose too early in treatment of patients with ectopic pregnancies. The possibility that non-responding patients could exist should be kept in mind.

引用

页码：311 / 319

页数：9

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