IMPROVED FLOW CYTOMETRIC METHOD FOR HLA-B27 TYPING

被引：11

作者：

JANSSEN, WCM ^{[1
]}

ROUWEN, JACN ^{[1
]}

HOFFMANN, JJML ^{[1
]}

机构：

[1] CATHARINA HOSP,DEPT CLIN LABS,POB 1350,5602 ZA EINDHOVEN,NETHERLANDS

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1992年 / 29卷

关键词：

IMMUNOFLUORESCENCE; LYMPHOCYTES; HUMAN LEUKOCYTE ANTIGEN;

D O I：

10.1177/000456329202900608

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7 antiserum. The flow cytometric method was evaluated by comparing the results with those obtained by the standard lymphocytotoxicity test and showed complete agreement in 107 selected patient samples.

引用

页码：663 / 667

页数：5

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