THE USE OF DAPHNIA-MAGNA FOR DETECTION OF OKADAIC ACID IN MUSSEL EXTRACTS

被引：7

作者：

VERNOUX, JP

LEBAUT, C

MASSELIN, P

MARAIS, C

BARON, B

CHOUMILOFF, R

PRONIEWSKI, F

NIZARD, G

BOHEC, M

机构：

[1] Laboratoire de Physiologie, Cellulaire et Moléculaire, Université de Caen Esplanade de la Paix, Caen Cedex

[2] Laboratoire Phycotoxines et Nuisances, Ifremer—Centre de Nantes, Nantes Cedex 01, 44037, BP 1049, Rue de l’Ile d’Yeu

[3] Laboratoire Municipal d’Hygiène du Havre, Le Havre

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1993年 / 10卷 / 05期

关键词：

DAPHNIA-MAGNA; OKADAIC ACID; MUSSELS; DSP;

D O I：

10.1080/02652039309374184

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Okadaic acid (OA), the main toxin responsible for diarrhetic shellfish poisoning (DSP) has toxicity for Daphnia magna (EC50 = 15 +/- 1.8 mug/l). A Daphnia bioassay was developed and used to analyse okadaic acid in mussel extracts. A linear correlation was found between OA concentration determined by the Daphnia bioassay and by HPLC assay (r = 0.85; p < 0.001). The Daphnia bioassay can measure OA levels 10 times below the threshold of the mouse bioassay method. It is an inexpensive sensitive tool which can be used in replacement of mouse bioassay for the screening of OA and some co-extracting toxins in mussel extracts.

引用

页码：603 / 608

页数：6

共 19 条

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