QUANTITATION OF CYTOKINE LEVELS IN SKIN-GRAFT DONOR SITE WOUND FLUID

被引：59

作者：

GRAYSON, LS ^{[1
]}

HANSBROUGH, JF ^{[1
]}

ZAPATASIRVENT, RL ^{[1
]}

DORE, CA ^{[1
]}

MORGAN, JL ^{[1
]}

NICOLSON, MA ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,MED CTR,DEPT SURG,200 W ARBOR DR,SAN DIEGO,CA 92103

来源：

BURNS | 1993年 / 19卷 / 05期

关键词：

D O I：

10.1016/0305-4179(93)90061-C

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

We quantified endogenous levels of multiple cytokines in skin graft donor site wounds in patients with small to moderate-sixed burn injuries. Thirteen patients aged 11 months to 61 years with mean TBSA burn of 4 +/- 1 per cent underwent placement of occlusive wound dressings on partial skin thickness donor site wounds. Fluid was aspirated from beneath the dressing on postoperative day 1 and every subsequent 24 h until no further fluid could be obtained. Interleukin-1 alpha (IL-1) and tumour necrosis factor alpha (TNF-alpha) levels were determined by enzyme-linked immunosorbent assay (ELISA). Epidermal growth factor (EGF), basic-fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) were measured by an enzyme immunoassay (ELA). We found substantial levels of EGF and TNF-alpha in the donor site wound fluid in all 13 patients, detectable levels of bFGF in five patients, and elevated levels of IL-1 in three patients. There were no detectable levels of these cytokines in normal human serum. In contrast, there were no measurable levels of PDGF in any patient's wound fluid, the mean level in serum was 1.5 ng/ml +/- 0.2 s.em. Studies of cytokines in the normal wound healing environment may help in the design of future therapies to augment wound healing.

引用

页码：401 / 405

页数：5

共 38 条

[1] Antoniades, Scher, Stiles, Purification of human platelet-derived growth factor, Proc. Natl. Acad. Sci. USA, 76, (1979)
[2] Barrandon, Green, Cell migration is essential for sustained growth of keratinocyte colonies: the roles of transforming growth factor alpha and epidermal growth factor, Cell, 50, (1987)
[3] Bauer, Cooper, Huang, Et al., Stimulation of in vitro human skin collagenase expression by platelet-derived growth factor, Proc. Natl. Acad. Sci. USA, 82, (1985)
[4] Brown, Curtsinger, Brightwell, Et al., Enhancement of epidermal regeneration by biosynthetic epidermal growth factor, J. Exp. Med., 163, (1986)
[5] Brown, Nanney, Griffen, Et al., Enhancement of wound healing by topical treatment with epidermal growth factor, N. Engl. J. Med., 321, (1989)
[6] Cohen, Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the newborn animal, J. Biol. Chem., 237, (1962)
[7] Collins, Stinnet-Smith, Rozengart, Platelet-derived growth factor treatment decreases the affinity of the epidermal growth receptors of Swiss 3T3 cells, J. Biol. Chem., 258, (1983)
[8] Cromack, Pierce, Mustoe, TGF-β and PDGF mediated tissue repair: identifying mechanisms of action using impaired and normal models of wound healing, Clinical and Experimental Approaches to Dermal and Epidermal Repair: Normal and Chronic Wounds, (1991)
[9] Dayer, Beutler, Cerami, Cachectin/TNF stimulates collagenase and prostaglandin E<sub>2</sub> production by human synovial cells and dermal fibroblasts, J. Exp. Med., 162, (1985)
[10] Derynck, Transforming growth factor-alpha: structure and biological activities, J. Cell. Biochem., 32, (1986)

← 1 2 3 4 →