USE OF SUCROSE GRADIENTS IN ISOLATION OF CYTOPLASMIC-POLYHEDROSIS VIRUS PARTICLES

被引:20
作者
HAYASHI, Y
BIRD, FT
机构
[1] Insect Pathology Research Institute, Department of Forestry and Rural Development, Sault Ste. Marie, Ont.
关键词
D O I
10.1016/0022-2011(68)90051-7
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Sucrose density gradients were used to isolate both free virus from midgut cells of the forest tent caterpillar, malacosoma disstria, infected with a cytoplasmic-polyhedrosis virus, and virus released when polyhedra were dissolved in weak alkali. Electron micrographs showed a high degree of purity of the virus preparations, and ultraviolet absorption spectra demonstrated typical nucleoproteins. © 1968.
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页码:40 / &
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