ENZYMES OF TRYPTOPHAN OPERON OF BACILLUS SUBTILIS

被引:59
作者
HOCH, SO
ANAGNOSTOPOULOS, C
CRAWFORD, IP
机构
[1] Scripps Clinic and Research Foundation La Jolla
[2] Centre de Genetique Moleculaire, C.N.R.S. Gif-sur-Yvette
关键词
D O I
10.1016/0006-291X(69)90700-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Methods for stabilizing the enzymes of the tryptophan synthetic pathway in extracts of Bacillus subtilis have been developed and are described. Examination of fourteen tryptophan auxotrophs showed only one enzymatic activity to be missing in each case. The size of the first four enzymes of the pathway was estimated by gel filtration. Synthesis of the enzymes of the pathway during derepression appears coordinate, as is the case in other bacteria where the structural genes for the pathway enzymes are clustered. © 1969.
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页码:838 / +
页数:1
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