NUCLEOTIDE-SEQUENCE OF THE 3 MAJOR EARLY PROMOTERS OF BACTERIOPHAGE-T7

I have determined the nucleotide sequences of the three major early promoters of bacteriophage T7 (Al, A2, A 3 ). The sequences confirm the two main homologies found between other known promoters for E. coil RNA polymerase (nucleoside triphosphate:RNA nucleotidyl transferase, E.C. 2.7.7.6 ). In particular, all three T7 promoters show a very good match with the -35 region homology; the A2 and A3 promoters share a 17 basepair sequence in this region. On the other hand, the match with the Pribnow Box homology is much less pronounced and different for each T7 promoter. © 1979 Information Retrieval Limited.