MICROPLATE ASSAY OF GLUTATHIONE S-TRANSFERASE ACTIVITY FOR RESISTANCE DETECTION IN SINGLE-MOSQUITO TRITURATES

被引：67

作者：

BROGDON, WG

BARBER, AM

机构：

[1] Malaria Branch, Division of Parasitic Diseases, Center for Infectious Disease, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, Atlanta

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 96卷 / 02期

关键词：

D O I：

10.1016/0305-0491(90)90385-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Optimum conditions are described for a simple, rapid microplate assay that measures glutathion s-transferase (GST) activity accurately and precisely in small portions of single mosquito homogenates. 2. 2. Up to 10 assay replicates were possible for individual adults and larvae. Concentration of GST activity in the head/thorax region allows blood-fed mosquitoes with abdomens removed to be used in assays. 3. 3. The method allows the use of GST activity as a biochemical character in comparative studies of populations. 4. 4. The microplate assay detects elevated GST activities associated with DDT resistance in Anopheles arabiensis. © 1990.

引用

页码：339 / 342

页数：4

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