ANTIBIOTIC INDUCED ELECTROPHORETIC MOBILITY SHIFTS OF DNA RESTRICTION FRAGMENTS

被引：11

作者：

LOUCKS, E ^{[1
]}

CHACONAS, G ^{[1
]}

BLAKESLEY, RW ^{[1
]}

WELLS, RD ^{[1
]}

VANDESANDE, JH ^{[1
]}

机构：

[1] UNIV WISCONSIN, DEPT BIOCHEM, DIV MED BIOCHEM, MADISON, WI 53706 USA

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 05期

基金：

美国国家卫生研究院; 英国医学研究理事会;

关键词：

D O I：

10.1093/nar/6.5.1869

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several antibiotics , netropsin, distamycin A, actinomycin D, Hoechst 33258 and olivomycin, which demonstrate base specificity in their DNA binding properties have been found to alter the electrophoretic mobility of DNA restriction fragments in native polyacrylamide gels. The antibiotics mostly reduced the migration of larger DM fragments, but netropsin and Hoechst 33258 were observed to increase the migration rate o f several DNA fragments of intermediate size. DNA fragments of similar molecular weight which comigrate as a single gel band can at times be separated as the result of differential mobility shifts promoted by antibiotic DNA complex formations. © 1979 Information Retrieval Limited.

引用

页码：1869 / 1879

页数：11

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