LOCALIZATION OF THE VIRUS NEUTRALIZING AND HEMAGGLUTININ EPITOPES OF E1 GLYCOPROTEIN OF RUBELLA-VIRUS

被引：42

作者：

CHAYE, H ^{[1
]}

CHONG, P ^{[1
]}

TRIPET, B ^{[1
]}

BRUSH, B ^{[1
]}

GILLAM, S ^{[1
]}

机构：

[1] CONNAUGHT CTR BIOTECHNOL RES,N YORK M2R 3T4,ONTARIO,CANADA

来源：

VIROLOGY | 1992年 / 189卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0042-6822(92)90572-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Current serological assays using whole rubella virus (RV) as a target antigen for detecting RV-specific antibodies fail to define specific RV proteins and antigenic determinants such as hemagglutinin (HA) and virus-neutralizing (VN) epitopes of rubella virus. A panel of El deletion mutants and a subset of E1-specific monoclonal antibodies (MAb) were used for the initial analysis of HA and VN epitopes of E1 glycoprotein. A peptide region (E1193 to E1269.) was found to contain HA and VN epitopes. Using both overlapping synthetic peptides and truncated fusion proteins within this region, the HA epitope defined by MAb 3D9F mapped to amino acid residues El214 to El240, while two VN epitopes defined by MAb 211391-1 and MAb 16A1 OE mapped to amino acid residues El 214 to E1233 and E1219 to E1233, respectively. The epitopes defined in this study are recognized by antibody whether or not the epitopes are glycosylated. © 1992.

引用

页码：483 / 492

页数：10

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