ONLINE ELECTROCHEMICAL REAGENT GENERATION FOR LIQUID-CHROMATOGRAPHY WITH LUMINOL-BASED CHEMILUMINESCENCE DETECTION

被引：6

作者：

STEIJGER, OM

DEJONG, GJ

HOLTHUIS, JJM

BRINKMAN, UAT

机构：

[1] DUPHAR BV,1381 CP WEESP,NETHERLANDS

[2] EUROCETUS BV,1105 BJ AMSTERDAM,NETHERLANDS

[3] FREE UNIV AMSTERDAM,DEPT ANALYT CHEM,1081 HV AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 557卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)87118-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An on-line method for the generation of electrochemical reagent for liquid chromatography, with luminol-based chemiluminescence detection, has been developed. An ESA Coulochem guard cell, equipped with a porous graphite working electrode, operated at -600 mV and inserted after the column, produces an oxidative reagent for the luminol-based reaction. This method has been compared with the conventional method with post-column addition of hydrogen peroxide as the oxidative reagent. With this novel method a detection limit of 0.15 pmol of ibuprofen (labelled with an isoluminol derivative) can be obtained, and a good alternative for post-column addition of hydrogen peroxide is presented.

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页码：13 / 21

页数：9

相关论文

共 26 条

[1]

Albreacht HO, 1928, Z PHYS CHEM-STOCH VE, V136, P321

[2] EVALUATION OF AN ISOLUMINOL CHEMI-LUMINESCENCE ASSAY FOR THE DETECTION OF HYDROPEROXIDES IN HUMAN-BLOOD PLASMA [J].

FREI, B ;

YAMAMOTO, Y ;

NICLAS, D ;

AMES, BN .

ANALYTICAL BIOCHEMISTRY, 1988, 175 (01) :120-130

[3] PHOTOOXYGENATION-CHEMILUMINESCENCE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETECTOR FOR THE DETERMINATION OF ALIPHATIC-ALCOHOLS, ALDEHYDES, ETHERS AND SACCHARIDES [J].

GANDELMAN, MS ;

BIRKS, JW .

JOURNAL OF CHROMATOGRAPHY, 1982, 242 (01) :21-31

[4] THE MECHANISM OF THE ELECTROGENERATED CHEMI-LUMINESCENCE OF LUMINOL IN AQUEOUS ALKALINE-SOLUTION [J].

HAAPAKKA, KE ;

KANKARE, JJ .

ANALYTICA CHIMICA ACTA, 1982, 138 (JUN) :263-275

[5] THE DETERMINATION OF A SMALL AMOUNT OF BIOLOGICAL CONSTITUENT BY THE USE OF CHEMILUMINESCENCE .7. AN ATTEMPT OF CYCLIC IMMUNOAFFINITY CHROMATOGRAPHY [J].

HARA, T ;

TORIYAMA, M ;

EBUCHI, T ;

IMAKI, M .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1986, 59 (07) :2368-2370

[6] THE DETERMINATION OF A SMALL AMOUNT OF BIOLOGICAL CONSTITUENT BY THE USE OF CHEMI-LUMINESCENCE .4. A HIGHLY SENSITIVE DETECTOR FOR THE HPLC OF PROTEIN [J].

HARA, T ;

TORIYAMA, M ;

EBUCHI, T .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1985, 58 (01) :109-114

[7] ROLE OF O2- IN CHEMILUMINESCENCE OF LUMINOL [J].

HODGSON, EK ;

FRIDOVICH, I .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1973, 18 (06) :451-455

[8] DETERMINATION OF COBALT AT PICOGRAM LEVELS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CHEMI-LUMINESCENCE DETECTION [J].

JONES, P ;

WILLIAMS, T ;

EBDON, L .

ANALYTICA CHIMICA ACTA, 1989, 217 (01) :157-163

[9] CHEMI-LUMINESCENCE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING N-(4-AMINOBUTYL)-N-ETHYLISOLUMINOL AS A PRECOLUMN LABELING REAGENT [J].

KAWASAKI, T ;

MAEDA, M ;

TSUJI, A .

JOURNAL OF CHROMATOGRAPHY, 1985, 328 (JUN) :121-126

[10] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF GLUCOSIDES (GLUCOSE CONJUGATES) WITH POST-COLUMN REACTION DETECTION COMBINING IMMOBILIZED ENZYME REACTORS AND LUMINOL CHEMI-LUMINESCENCE [J].

KOERNER, PJ ;

NIEMAN, TA .

JOURNAL OF CHROMATOGRAPHY, 1988, 449 (01) :217-228