THE CA2+-RELEASE CHANNEL RYANODINE RECEPTOR IS LOCALIZED IN JUNCTIONAL AND CORBULAR SARCOPLASMIC-RETICULUM IN CARDIAC-MUSCLE

被引:128
作者
JORGENSEN, AO [1 ]
SHEN, ACY [1 ]
ARNOLD, W [1 ]
MCPHERSON, PS [1 ]
CAMPBELL, KP [1 ]
机构
[1] UNIV IOWA,COLL MED,PROGRAM NEUROSCI,DEPT PHYSIOL & BIOPHYS,HOWARD HUGHES MED INST,IOWA CITY,IA 52242
关键词
D O I
10.1083/jcb.120.4.969
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The subcellular distribution of the Ca2+-release channel/ryanodine receptor in adult rat papillary myofibers has been determined by immunofluorescence and immunoelectron microscopical studies using affinity purified antibodies against the ryanodine receptor. The receptor is confined to the sarcoplasmic reticulum (SR) where it is localized to interior and peripheral junctional SR and the corbular SR, but it is absent from the network SR where the SR-Ca2+-ATPase and phospholamban are densely distributed. Immunofluorescence labeling of sheep Purkinje fibers show that the ryanodine receptor is confined to discrete foci while the SR-Ca2+-ATPase is distributed in a continuous network-like structure present at the periphery as well as throughout interior regions of these myofibers. Because Purkinje fibers lack T-tubules, these results indicate that the ryanodine receptor is localized not only to the peripheral junctional SR but also to corbular SR densely distributed in interfibrillar spaces of the I-band regions. We have previously identified both corbular SR and junctional SR in cardiac muscle as potential Ca2+-storage/Ca2+-release sites by demonstrating that the Ca2+ binding protein calsequestrin and calcium are very densely distributed in these two specialized domains of cardiac SR in situ. The results presented here provide strong evidence in support of the hypothesis that corbular SR is indeed a site of Ca2+-induced Ca2+ release via the ryanodine receptor during excitation contraction coupling in cardiac muscle. Furthermore, these results indicate that the function of the cardiac Ca2+-release channel/ryanodine receptor is not confined to junctional complexes between SR and the sarcolemma.
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页码:969 / 980
页数:12
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