IDENTIFICATION OF THE IRON-CARBONYL STRETCH IN DISTAL HISTIDINE MUTANTS OF CARBONMONOXYMYOGLOBIN

被引：41

作者：

LING, JH

LI, TS

OLSON, JS

BOCIAN, DF

机构：

[1] UNIV CALIF RIVERSIDE, DEPT CHEM, RIVERSIDE, CA 92521 USA

[2] RICE UNIV, DEPT BIOCHEM & CELL BIOL, HOUSTON, TX 77251 USA

[3] RICE UNIV, WM KECK CTR COMPUTAT BIOL, HOUSTON, TX 77251 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1994年 / 1188卷 / 03期

关键词：

MYOGLOBIN; GENETIC MODIFICATION; RESONANCE RAMAN; INFRARED; CONFORMATIONAL SUBSTATE;

D O I：

10.1016/0005-2728(94)90063-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Soret-excitation resonance Raman (RR) spectra are reported for six distal histidine mutants of carbonmonoxymyoglobin including H64A, H64V, H64L, H64I, H64W, and H64W/L29F. Based on (CO)-C-13 isotope shifts, the iron-carbonyl stretching vibrations are unambiguously identified. The correct assignment of these modes eliminates the differences in the conformational substate occupations predicted by the RR versus IR data.

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收藏

页码：417 / 421

页数：5

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